LDHB Protein (AA 1-334)

Produktnummer ABIN6387839

Produktdetails

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-334

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Sequenz: MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL

Reinheit: > 90 % by SDS - PAGE

Endotoxin-Niveau: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 300 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl (  pH 8.0) containing 10 % glycerol, 1  mM DTT

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Andere Bezeichnung: LDHB (LDHB Produkte)

Synonyme: LDH-B Protein, LDH-H Protein, LDHBD Protein, TRG-5 Protein, AI790582 Protein, H-Ldh Protein, Ldh-2 Protein, Ldh2 Protein, ldh-h Protein, ldh2 Protein, ldhb Protein, ldhba Protein, trg-5 Protein, LDHB Protein, LDH-B-A Protein, wu:fa16d07 Protein, wu:fa20c10 Protein, wu:fa96a08 Protein, LDH-B-B Protein, zgc:92882 Protein, lactate dehydrogenase B Protein, lactate dehydrogenase B S homeolog Protein, lactate dehydrogenase Ba Protein, lactate dehydrogenase Bb Protein, LDHB Protein, Ldhb Protein, ldhb.S Protein, ldhb Protein, ldhba Protein, ldhbb Protein

Hintergrund: LDHB, also known as L-lactate dehydrogenase B chain isoform LDHB, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate. The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors. Recombinant human LDHB protein was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 36.6kDa (334aa) confirmed by MALDI-TOF

NCBI Accession: NP_002291

UniProt: P07195

Pathways: Warburg Effekt