zgc:73302 Protein, wu:fj61c06 Protein, VAMP2 Protein, vamp2-a Protein, xsybi Protein, Syb-2 Protein, Syb2 Protein, sybII Protein, RATVAMPB Protein, RATVAMPIR Protein, SYB Protein, SYB2 Protein, VAMP-2 Protein, syb2 Protein, vamp2 Protein, xsybii Protein, vesicle-associated membrane protein 2 Protein, vesicle associated membrane protein 2 Protein, vesicle associated membrane protein 2 S homeolog Protein, vesicle-associated membrane protein 2 (synaptobrevin 2) Protein, vesicle associated membrane protein 2 L homeolog Protein, vamp2 Protein, VAMP2 Protein, vamp2.S Protein, Vamp2 Protein, vamp2.L Protein
Hintergrund
VAMP2, also known as vehicle-associated membrane protein 2 isoform 1, which is an integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal. VAMP2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25. Recombinant human VAMP2 protein, fused to His-tag at N-terminus, was expressed in E,coli and using conventional chromatography.