GZMB Protein (AA 19-247) (His tag)

Produktnummer ABIN7529398

Produktdetails

Target: GZMB (Granzyme B (GZMB))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 19-247

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses GZMB Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: GEIIGGHEAK PHSRPYMAYL MIWDQKSLKR CGGFLIRDDF VLTAAHCWGS SINVTLGAHN IKEQEPTQQF IPVKRPIPHP AYNPKNFSND IMLLQLERKA KRTRAVQPLR LPSNKAQVKP GQTCSVAGWG QTAPLGKHSH TLQEVKMTVQ EDRKCESDLR HYYDSTIELC VGDPEIKKTS FKGDSGGPLV CNKVAQGIVS YGRNNGMPPR ACTKVSSFVH WIKKTMKRYH HHHHH

Reinheit: > 95 % by SDS - PAGE

Endotoxin-Niveau: < 1.0 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 7,000 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 20 % glycerol, 1  mM DTT.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: GZMB (Granzyme B (GZMB))

Andere Bezeichnung: GZMB (GZMB Produkte)

Synonyme: CCPI Protein, CGL-1 Protein, CGL1 Protein, CSP-B Protein, CSPB Protein, CTLA1 Protein, CTSGL1 Protein, HLP Protein, SECT Protein, granzyme B Protein, GZMB Protein

Hintergrund: GZMB, also known as granzyme B isoform 1, is member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. Tis protein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. Also, it processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. Recombinant human GZMB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molekulargewicht: 26.5kDa (235aa) 28-40kDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_004122

UniProt: P10144

Pathways: Apoptose, Caspase Kaskade in der Apoptose