Biglycan Protein (BGN) (AA 38-368) (His tag)

Produktnummer ABIN7529401

Produktdetails

Target: Biglycan (BGN)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 38-368

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses Biglycan Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: ADPDEEASGA DTSGVLDPDS VTPTYSAMCP FGCHCHLRVV QCSDLGLKSV PKEISPDTTL LDLQNNDISE LRKDDFKGLQ HLYALVLVNN KISKIHEKAF SPLRKLQKLY ISKNHLVEIP PNLPSSLVEL RIHDNRIRKV PKGVFSGLRN MNCIEMGGNP LENSGFEPGA FDGLKLNYLR ISEAKLTGIP KDLPETLNEL HLDHNKIQAI ELEDLLRYSK LYRLGLGHNQ IRMIENGSLS FLPTLRELHL DNNKLARVPS GLPDLKLLQV VYLHSNNITK VGVNDFCPMG FGVKRAYYNG ISLFNNPVPY WEVQPATFRC VTDRLAIQFG NYKKHHHHHH

Reinheit: > 90 % by SDS - PAGE

Endotoxin-Niveau: < 1.0 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured in inhibit the cell growth using 3T3-L1 mouse embryonic fibroblast adipose-like cells. The ED50 for this effect is less or equal to 20 ug/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: Biglycan (BGN)

Andere Bezeichnung: BGN (BGN Produkte)

Synonyme: DSPG1 Protein, PG-S1 Protein, PGI Protein, SLRR1A Protein, BG Protein, BSPG1 Protein, bgn Protein, zgc:91989 Protein, dspg1 Protein, pg-s1 Protein, slrr1a Protein, xbgn Protein, BGN Protein, zgc:77123 Protein, DKFZp468I2427 Protein, biglycan Protein, biglycan L homeolog Protein, biglycan a Protein, biglycan S homeolog Protein, biglycan b Protein, BGN Protein, Bgn Protein, bgn.L Protein, bgna Protein, bgn.S Protein, bgn Protein, bgnb Protein

Hintergrund: BGN, also known as biglycan preproprotein, is a small leucine-rich repeat proteoglycan (SLRP). It plays an important role in the development and maintenance of many tissues. Core protein of this protein binds to the growth factors BMP-4 and influences its bioactivity. This protein is also involved in collagen fiber assembly. Recombinant human BGN protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molekulargewicht: 38.3kDa (340aa) 40-57KDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_001702

UniProt: P21810

Pathways: Glycosaminoglycan Metabolic Process