BAG1 Protein (GST tag)

Produktnummer ABIN6699543

Hersteller Produkt- Nr.: 009-001-r46s

Produktdetails

Target: BAG1 (BCL2-Associated Athanogene (BAG1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses BAG1 Protein ist gelabelt mit GST tag.

Applikation: Western Blotting (WB)

Hersteller Produkt- Nr.: 009-001-r46s

Hersteller: Rockland

Verwendungszweck: BAG1 recombinant protein-GST fusion protein

Aufreinigung: Recombinant human BAG1 (72-end) was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.

Reinheit: >85%

Anwendungsinformationen

Applikationshinweise:

Western_Blot_Dilution: User Optimized

Application_Note: BAG1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 66 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 μg/μL

Buffer: BAG1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: BAG1 (BCL2-Associated Athanogene (BAG1))

Andere Bezeichnung: BAG1 (BAG1 Produkte)

Hintergrund:

Synonyms: BAG family molecular chaperone regulator 1 (BAG-1) (Bcl-2-associated athanogene 1), HAP, RAP46

Background: BAG1 (also known as BCL2-associated athanogene) is a membrane protein rich in glutamic acid residues that binds to BCL2 and blocks apoptosis or programmed cell death (1). The BAG1-BCL2 complex enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms (2). Overexpression of BAG1 in 3T3 fibroblasts prevents apoptosis in the presence of low serum. BAG1 has also been shown to interact with activated glucocorticoid, androgen, estrogen and progesterone receptors. Binding to these receptors by BAG1 is dependent on receptor activation. BAG1 Protein is ideal for investigators involved in Signaling Proteins, Apoptosis Proteins, AKT/PKB Pathway, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, and Neurobiology research.

NCBI Accession: NM_004323