BDNF Protein

Produktnummer ABIN6699554

Hersteller Produkt- Nr.: 009-001-c27

Produktdetails

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Hersteller Produkt- Nr.: 009-001-c27

Hersteller: Rockland

Verwendungszweck: BDNF Human Recombinant Protein

Aufreinigung: Purity was determined to be greater than 97% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE against a known standard. Endotoxin level as determined by kinetic LAL analysis: 0.05 EUs/μg protein.

Reinheit: 97,00%

Endotoxin-Niveau: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: Proliferation of a neuroblastoma cell line stably expressing TrkB (BR6) ED50 ≤ 40 ng/mL (≥ 2.5 x 10^4 units/mg).

Anwendungsinformationen

Applikationshinweise: BDNF has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-BDNF in immunological assays.

Kommentare:

Suggested_Applications: Other

Other_Performance_Data:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Konzentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Referenzen

Chowdhury, Collins, Gell, Perry, Breadmore, Shigdar, King: "Isolation and Identification of the High-Affinity DNA Aptamer Target to the Brain-Derived Neurotrophic Factor (BDNF)." in: ACS chemical neuroscience, Vol. 15, Issue 2, pp. 346-356, (2024) (PubMed).

Antigendetails

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Andere Bezeichnung: BDNF (BDNF Produkte)

Hintergrund:

Synonyms: Brain-derived neurotrophic factor, Abrineurin cytokine

Background: BDNF is a member of the nerve growth factor family of trophic factors. In the brain BDNF has a trophic action on retinal, cholinergic, and dopaminergic neurons, and in the peripheral nervous system it acts on both motor and sensory neurons. Some protein domains of BDNF are identical with those of NGF and another neurotrophic factor, designated NT-3 (neurotrophin-3). It exists as monomers and homodimers, and binds to NTRK2/TRKB. Polyclonal antibodies raised against murine NGF have been shown to cross-react with both NT-3 and BDNF. The propeptide is N-glycosylated and glycosulfated. BDNF is converted into mature BDNF by plasmin (PLG). It is expressed in brain, and highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum, it is also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. Recombinant Human Brain-Derived Neurotrophic Factor produced in E.coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids and having a total molecular mass of 26,984 Daltons.

UniProt: P23560

Pathways: RTK Signalweg, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity