CCL5 Protein

Produktnummer ABIN6699634

Hersteller Produkt- Nr.: 009-001-c20-0005

Produktdetails

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Hersteller Produkt- Nr.: 009-001-c20-0005

Hersteller: Rockland

Verwendungszweck: Human RANTES (CCL5) Recombinant Protein

Aufreinigung: RANTES (CCL5) purity was determined to be greater than 98% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Reinheit: 98,00%

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to chemoattract human monocytes, neutrophils, THP-1 or primary T cells at 1-8 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Other: User Optimized

Application_Note: RANTES Recombinant Protein has been tested by biologic activity and is suitable as a control for polyclonal or monoclonal anti-RANTES in immunological assays.

Kommentare:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Andere Bezeichnung: CCL5 (CCL5 Produkte)

Hintergrund:

Synonyms: EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228 (TCP228), T-cell-specific protein RANTES

Background: Regulated Upon Activation Normal T cell Express Sequence (RANTES), also called CCL5, is a chemokine produced by T cells three to five days after activation. RANTES is a promiscuous chemokine that signals through several G protein-coupled receptors, CCR5, CCR3, CCR1 and US28 (a viral receptor encoded by human CMV). The main function of RANTES is to recruit immune cells to the site of inflammation. Recombinant human RANTES is a non-glycosylated protein, containing 68 amino acids, with a molecular weight of 7.8 kDa.

UniProt: P13501

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration