CCL7 Protein

Produktnummer ABIN6699647

Hersteller Produkt- Nr.: 010-001-v76-0010

Produktdetails

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Hersteller Produkt- Nr.: 010-001-v76-0010

Hersteller: Rockland

Verwendungszweck: Mouse Monocyte Chemotactic Protein-3 (CCL7) Recombinant Protein

Aufreinigung: Monocyte Chemotactic protein-3 (CCL7) purity was determined to be greater than 98% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Reinheit: 98,00%

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by its ability to chemoattract THP-1 cells at 10-100 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Other: User Optimized

Application_Note: Monocyte Chemotactic Protein-3 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Monocyte Chemotactic Protein-3 in immunological assays.

Kommentare:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Andere Bezeichnung: Ccl7 (CCL7 Produkte)

Hintergrund:

Synonyms: Intercrine/chemokine MARC, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3 (MCP-3), Protein FIC, Small-inducible cytokine A7

Background: Monocyte Chemotactic Protein 3 (MCP-3), also called CCL7, is produced by macrophages and some tumor cell lines. MCP-3 signals through three different G protein-coupled receptors, CCR1, CCR2, and CCR3. CCL7 chemoattracts monocytes and can regulate macrophage function. Recombinant mouse MCP-3 is a non-glycosylated protein, containing 74 amino acids, with a molecular weight of 8.5 kDa.

UniProt: Q03366