G-CSF Protein
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- Target Alle G-CSF (CSF3) Proteine anzeigen
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
- Protein-Typ
- Recombinant
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Applikation
- SDS-PAGE (SDS)
- Hersteller Produkt- Nr.
- 009-001-u93-0010
- Hersteller
- Rockland
- Verwendungszweck
- Human Granulocyte Colony Stimulating Factor Recombinant Protein
- Aufreinigung
- Granulocyte Colony Stimulating Factor purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
- Reinheit
- 95,00%
- Endotoxin-Niveau
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the dose-dependent proliferation of mouse NFS-60 and is typically 10-60 pg/mL.
- Top Product
- Discover our top product CSF3 Protein
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- Applikationshinweise
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Other: User Optimized
Application_Note: Granulocyte Colony Stimulating Factor Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Granulocyte Colony Stimulating Factor in immunological assays.
- Kommentare
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- Konzentration
- 0.1 mg/mL
- Buffer
- Lyophilized in 20 mM acetic acid, 50 nM sodium chloride.
- Konservierungsmittel
- Without preservative
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Haltbarkeit
- 6 months
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- Target
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
- Andere Bezeichnung
- CSF3 (CSF3 Produkte)
- Hintergrund
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Synonyms: CSF-3, MGI-1G, GM-CSFβ, pluripoietin
Background: Granulocyte-Colony Stimulating Factor (G-CSF) is a growth factor that is considered the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF, while in epithelial, endothelial, and fibroblastic cells, secretion of G-CSF is induced by IL-17. Human and mouse G-CSF are cross-reactive. Recombinant human G-CSF is a non-glycosylated protein, containing 175 amino acids, with a molecular weight of 18.8 kDa.
- UniProt
- P09919
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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