Cyclin C Protein (CCNC) (GST tag)

Produktnummer ABIN6699737

Hersteller Produkt- Nr.: 009-001-r77s

Produktdetails

Target: Cyclin C (CCNC)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Insect cells (Sf9)

Aufreinigungstag / Konjugat: Dieses Cyclin C Protein ist gelabelt mit GST tag.

Applikation: Western Blotting (WB)

Hersteller Produkt- Nr.: 009-001-r77s

Hersteller: Rockland

Verwendungszweck: CyclinC recombinant protein-GST fusion protein

Aufreinigung: Recombinant full-length human CyclinC was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.

Reinheit: >70%

Anwendungsinformationen

Applikationshinweise:

Western_Blot_Dilution: User Optimized

Application_Note: CyclinC Protein is suitable for use in Western Blot. Expect a band approximately ~ 55 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.05 μg/μL

Buffer: CyclinC Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Cyclin C (CCNC)

Andere Bezeichnung: CCNC (CCNC Produkte)

Hintergrund:

Synonyms: CCNC, CycC, Cyclin-C, SRB11 homolog, hSRB11

Background: Cyclin C is a member of the cyclin family of proteins and interacts with the cyclin-dependent kinase 8 (CDK8) and induces the phosphorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The mRNA levels of Cyclin C fluctuate during the cell cycle and peak at the G1 phase. Cyclin C acts as a critical regulator of the G0/G1 transition of human Hematopoietic stem cells (1). Cyclin C can also combine with CDK3 to stimulate Rb phosphorylation at S807/811 during the G0/G1 transition, and this phosphorylation is required for cells to exit G0 efficiently (2). Cyclin C Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.

NCBI Accession: NM_005190

Pathways: Zellzyklus, Regulation of Lipid Metabolism by PPARalpha