FGF4 Protein

Produktnummer ABIN6699859

Hersteller Produkt- Nr.: 009-001-u76-0025

Produktdetails

Target: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Hersteller Produkt- Nr.: 009-001-u76-0025

Hersteller: Rockland

Verwendungszweck: Human Fibroblast Growth Factor-4 Recombinant Protein

Aufreinigung: Fibroblast Growth Factor-4 purity was determined to be greater than 98% as determined by analysis by reducing and non-reducing SDS-pAGE.

Reinheit: 98,00%

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by its ability to induce the proliferation of mouse NR6R-3T3 fibroblasts and is typically 0.25-1.25 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Other: User Optimized

Application_Note: Fibroblast Growth Factor-4 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Fibroblast Growth Factor-4 in immunological assays.

Kommentare:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 25 μL (25-250 μL)

Buffer: Lyophilized in 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Andere Bezeichnung: FGF4 (FGF4 Produkte)

Hintergrund:

Synonyms: Transforming protein KS3, Heparin-binding growth factor (HBGF-4), Heparin secretory-transforming protein 1 (HST-1)

Background: Fibroblast Growth Factor 4 (FGF-4) is a growth factor predominantly expressed during embryonic development, playing a key role in limb development. In culture, FGF-4 has been shown to be an important regulator of growth for stem cells, fibroblasts and endothelial cells. Unglycosylated FGF-4 is N-terminally cleaved into 13 kDa or 15 kDa proteins that are more active than the precursor 19 kDa protein, Bellosa P, et al. (1993). Human FGF-4 shares high homology and cross-reactivity with the mouse protein. Recombinant human FGF-4 is a non-glycosylated protein containing 140 amino acids, with a total molecular weight of 15 kDa.

UniProt: P08620

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance