GDF5 Protein

Produktnummer ABIN6699913

Hersteller Produkt- Nr.: 009-001-u94-0050

Produktdetails

Target: GDF5 (Growth Differentiation Factor 5 (GDF5))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Hersteller Produkt- Nr.: 009-001-u94-0050

Hersteller: Rockland

Verwendungszweck: Human Growth and Differentiation Factor-5 Recombinant Protein

Aufreinigung: Growth and Differentiation Factor-5 purity was determined to be greater than 98% as determined by analysis by HpLC, UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Reinheit: 98,00%

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to induce alkaline phosphatase activity in ATDC5 cells is typically 0.1-1 μg/mL.

Anwendungsinformationen

Applikationshinweise:

Other: User Optimized

Application_Note: Growth and Differentiation Factor-5 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Growth and Differentiation Factor-5 in immunological assays.

Kommentare:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 50μL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: GDF5 (Growth Differentiation Factor 5 (GDF5))

Andere Bezeichnung: GDF5 (GDF5 Produkte)

Hintergrund:

Synonyms: Cartilage-derived morphogenetic protein 1

Background: Growth/Differentiation Factor 5 (GDF-5) is a growth factor that regulates cell proliferation and differentiation in embryonic and adult tissues. GDF-5 is part of the TGF family of proteins and is closely related to the BMP family of proteins. Recombinant human GDF-5 is a non-glycosylated homodimer, containing two 120 amino acids chains, with a total molecular weight of 27.4 kDa. To enable bacterial expression the N-terminal sequence of Ala-Pro-Leu-Thr was replaced with a Lys.

UniProt: P43026