FGF4 Protein

Produktnummer ABIN6699931

Hersteller Produkt- Nr.: 009-001-f50s

Produktdetails

Target: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB)

Hersteller Produkt- Nr.: 009-001-f50s

Hersteller: Rockland

Verwendungszweck: HSF1 recombinant protein

Aufreinigung: Recombinant full length human HSF1 was expressed in E.coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Reinheit: >90%

Anwendungsinformationen

Applikationshinweise:

Western_Blot_Dilution: User Optimized

Other: Kinase Assay-User Optimized

Application_Note: HSF1 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~96 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 μg/μL

Buffer: HSF1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Andere Bezeichnung: FGF4 (FGF4 Produkte)

Hintergrund:

Synonyms: Heat Shock Transcription Factor 1, HSTF1, Fibroblast growth factor 4, Heparin secretory-transforming protein 1, Heparin-binding growth factor 4, Transforming protein KS3, FGF-4, HSTF-1, HST, Transforming protein KS3

Background: HSF1 is a member of the heat shock transcription factor family. Protein-damaging stress lead to the activation of HSF1 which binds to upstream regulatory sequences in the promoters of heat shock genes leading to enhanced heat shock gene expression (1). The activation of HSF1 proceeds through a multi-step pathway, involving a monomer-to-trimer transition, nuclear accumulation and extensive posttranslational modifications. HSF1 activity is regulated at different levels by heat shock proteins and co-chaperones and is modulated further by a number of mechanisms involving other stress-regulated aspects of cell metabolism (2). HSF1 Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Cancer, Cardiovascular Disease, Cellular Stress, Inflammation, and JNK/SAPK Pathway research.

NCBI Accession: NM_005526

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance