Interferon gamma Protein (IFNG)

Produktnummer ABIN6699975

Hersteller Produkt- Nr.: 009-001-v20-0020

Produktdetails

Target: Interferon gamma (IFNG)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Hersteller Produkt- Nr.: 009-001-v20-0020

Hersteller: Rockland

Verwendungszweck: Human Interferon-gamma Recombinant Protein

Aufreinigung: Interferon-gamma purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Reinheit: 95,00%

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is measured in antiviral assays using virally infected L929 cells and is typically less than 0.1 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Other: User Optimized

Application_Note: Interferon-gamma Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Interferon-gamma in immunological assays.

Kommentare:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Konzentration: 0.1 mg/mL

Buffer: Lyophilized from an aqueous solution containing 10 mM Sodium Phosphate, 50 mM Sodium Chloride, pH 7.5.

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: Interferon gamma (IFNG)

Andere Bezeichnung: IFNG (IFNG Produkte)

Hintergrund:

Synonyms: Type II interferon, T cell interferon, Immune Interferon, MAF

Background: Interferon gamma, IFNγ, is a major immune-modulating molecule produced mainly by T cells and natural killer cells activated by antigens, mitogens or alloantigens. Most immune cells express IFN-γ receptors and respond to IFNγ-induced signaling by up-regulating MHC class I expression. Human IFNγ does not show cross-reactivity with mouse. Recombinant human IFNγ is a non-glycosylated protein, containing 144 amino acids, with a molecular weight of 16.88 kDa.

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie