IL-3 Protein

Produktnummer ABIN6700114

Hersteller Produkt- Nr.: 010-001-b92-0010

Produktdetails

Target: IL-3 (Interleukin 3 (IL-3))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Hersteller Produkt- Nr.: 010-001-b92-0010

Hersteller: Rockland

Verwendungszweck: Mouse Interleukin-3 Recombinant Protein

Aufreinigung: Interleukin-3 purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Reinheit: 97,00%

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent stimulation of mouse M-NFS-60 cells and is typically less than 0.05 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Other: User Optimized

Application_Note: Mouse Interleukin-3 Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Mouse Interleukin-3 in immunological assays.

Kommentare:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: IL-3 (Interleukin 3 (IL-3))

Andere Bezeichnung: Il3 (IL-3 Produkte)

Hintergrund:

Synonyms: Mast cell growth factor (MCGF), Multipotential colony-stimulating factor, Multi-CSF, Hematopoietic growth factor, HCGF, P-cell stimulation factor

Background: Interleukin-3 (IL-3) is a cytokine produced by activated T cells and mast cells. IL-3 is able to induce the differentiation of hematopoietic stem cells to precursor cells of myeloid lineage (erythrocytes, megakaryocytes, granulocytes, dendritic cells and monocytes). IL-3 also has functions in the nervous system and appears to be important in several chronic inflammatory diseases. Recombinant mouse IL-3 is a non-glycosylated protein, containing 135 amino acids, with a molecular weight of 15.1 kDa.

UniProt: P01586

Pathways: JAK-STAT Signalweg, Regulation of Carbohydrate Metabolic Process, Autophagie