IL-4 Protein

Produktnummer ABIN6700140

Hersteller Produkt- Nr.: 010-001-b93-0005

Produktdetails

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Hersteller Produkt- Nr.: 010-001-b93-0005

Hersteller: Rockland

Verwendungszweck: Mouse Interleukin-4 Recombinant Protein

Aufreinigung: Interleukin-4 purity was determined to be greater than 95% as determined by HpLC and by reducing and non-reducing SDS-pAGE.

Reinheit: 95,00%

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by dose-dependent induction of HT-2 cell proliferation and is typically less than 2 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Other: User Optimized

Application_Note: Interleukin-4 Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-4 in immunological assays.

Kommentare:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Andere Bezeichnung: Il4 (IL4 Produkte)

Hintergrund:

Synonyms: B cell Stimulating Factor 1 (BSF-1), BCDF, BCGF, B-cell IgG differentiation factor, B-cell growth factor 1, IGG1 induction factor, Lymphocyte stimulatory factor 1

Background: Interleukin-4 (IL-4) is an immuno-modulatory cytokine, produced primarily by a subgroup of helper T cells, known as Th2. IL-4 is known to induce naive helper T cells to differentiate to Th2 cells, which subsequently produce more IL-4. Th2 cells regulate a wide variety of other immune cells including, B cells, where it has the ability to promote IgG to IgE isotype switching. Recombinant mouse IL-4 is a non-glycosylated protein, containing 121 amino acids, with a molecular weight of 13.5 kDa.

UniProt: P07750

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation