KIT Ligand Protein (KITLG)

Produktnummer ABIN6700197

Hersteller Produkt- Nr.: 010-001-w20-0100

Produktdetails

Target: KIT Ligand (KITLG)

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Hersteller Produkt- Nr.: 010-001-w20-0100

Hersteller: Rockland

Verwendungszweck: Mouse Stem Cell Factor Recombinant Protein

Aufreinigung: Stem Cell Factor purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Reinheit: 98,00%

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of human TF-1 cells and is typically less than 10 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Other: User Optimized

Application_Note: Stem Cell Factor Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Stem Cell Factor in immunological assays.

Kommentare:

Suggested_Applications: Cellular Assay, IHC, WB

Other_Performance_Data:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Konzentration: 0.1 mg/mL

Buffer: Lyophilized with 20 mM sodium phosphate, pH 6.5.

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Referenzen

Turnis, Kaminska, Smith, Kartchner, Vogel, Laxton, Ashmun, Ney, Opferman: "Requirement for antiapoptotic MCL-1 during early erythropoiesis." in: Blood, Vol. 137, Issue 14, pp. 1945-1958, (2021) (PubMed).

Antigendetails

Target: KIT Ligand (KITLG)

Andere Bezeichnung: Kitlg (KITLG Produkte)

Hintergrund:

Synonyms: c-Kit Ligand, Hematopoietic growth factor KL, KL, Steel Factor, Stem cell factor (SCF), Mast cell growth factor(MGF)

Background: Stem Cell Factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor known as c-Kit (CD117) and is thought to play a critical role in the maintenance or survival of hematopoietic stem cells. Human SCF shows no activity on murine cells, but murine and rat SCF are active on human cells. Recombinant mouse SCF is a non-glycosylated protein, containing 164 amino acids, with a molecular weight of 18.3 kDa.

UniProt: P20826

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung