Galectin 3 Protein (LGALS3)

Produktnummer ABIN6700207

Hersteller Produkt- Nr.: 009-001-u92-0050

Produktdetails

Target: Galectin 3 (LGALS3)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Hersteller Produkt- Nr.: 009-001-u92-0050

Hersteller: Rockland

Verwendungszweck: Human Galectin-3 Recombinant Protein

Aufreinigung: Galectin-3 purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Reinheit: 97,00%

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to induce chemotaxis of human PBMCs at concentrations ranging from 2-220 μg/mL.

Anwendungsinformationen

Applikationshinweise:

Other: User Optimized

Application_Note: Galectin-3 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Galectin-3 in immunological assays.

Kommentare:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 50μL

Buffer: Lyophilized in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: Galectin 3 (LGALS3)

Andere Bezeichnung: LGALS3 (LGALS3 Produkte)

Hintergrund:

Synonyms: 35 kDa lectin, Galactose-specific lectin-3, Galactoside-binding protein (GALBP), IgE-binding protein, Laminin-binding protein, MAC2, L-29 CPB-35

Background: Galectin-3 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-3 is expressed by a wide range of cell types including activated T cells, tumor cells, macrophages, osteoclasts, fibroblasts and epithelial cells and interacts with β-galactoside sugar moieties. Galectin-3 is associated with cancer, heart failure, stroke and inflammation. Human and mouse Galectin-3 share an 80 % homology by amino acid sequence. Recombinant human Galectin-3 is a non-glycosylated protein, containing 250 amino acids, with a molecular weight of 26 kDa.

UniProt: P17931

Pathways: RTK Signalweg