Moesin Protein (MSN) (GST tag)

Produktnummer ABIN6700268

Hersteller Produkt- Nr.: 009-001-s50

Produktdetails

Target: Moesin (MSN)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses Moesin Protein ist gelabelt mit GST tag.

Applikation: Western Blotting (WB)

Hersteller Produkt- Nr.: 009-001-s50

Hersteller: Rockland

Verwendungszweck: Moesin recombinant protein-GST fusion protein

Aufreinigung: Recombinant human Moesin (410-end) was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Reinheit: >95%

Anwendungsinformationen

Applikationshinweise:

Western_Blot_Dilution: User Optimized

Other: Kinase Assay-User Optimized

Application_Note: Moesin Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~50 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 μg/μL

Buffer: Moesin Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Moesin (MSN)

Andere Bezeichnung: MSN (MSN Produkte)

Hintergrund:

Synonyms: MSN, Moesin Membrane-organizing extension spike protein

Background: Moesin (or membrane-organizing extension spike protein) belongs to ERM family that modulates epithelial integrity by regulating cell-signalling events that affect actin organization and polarity (1). The effects of Moesin on epithelial cells appear to result from inhibition of Rho signaling. ERM proteins serve a structural role in linkage of the cytoskeleton to the plasma membrane and the rescue of cells lacking Moesin by modulation of Rho signaling indicates that inhibition of Rho activity may be a more critical function of Moesin. The negative feedback loop produced by Rho's activation of ERM may be an important mechanism that prevents the excessive migratory and invasive properties characteristic of metastatic cancer cells (2). Moesin Protein is ideal for investigators involved in Signaling Reagents, Protein Substrates, Inflammation, and PKA/PKC Pathway research.

NCBI Accession: NM_002444

Pathways: Asymmetric Protein Localization