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MGMT Protein (His tag)

Rockland 009-001-s47s MGMT Spezies: Human Wirt: Insect cells (Sf9) Recombinant >95% WB
Rockland
Produktnummer ABIN6700328
Hersteller Produkt- Nr.: 009-001-s47s
  • Target Alle MGMT Proteine anzeigen
    MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
    Protein-Typ
    Recombinant
    Spezies
    • 8
    • 2
    • 2
    • 1
    Human
    Quelle
    • 5
    • 3
    • 2
    • 2
    • 1
    Insect cells (Sf9)
    Aufreinigungstag / Konjugat
    Dieses MGMT Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB)
    Hersteller Produkt- Nr.
    009-001-s47s
    Hersteller
    Rockland
    Verwendungszweck
    MGMT recombinant protein-HIS Epitope
    Aufreinigung
    Recombinant full length human MGMT was expressed by baculovirus in Sf9 insect cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.
    Reinheit
    >95%
    Top Product
    Discover our top product MGMT Protein
  • Applikationshinweise

    Western_Blot_Dilution: User Optimized

    Application_Note: MGMT Protein is suitable for use in Western Blot. Expect a band approximately ~25 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.1 μg/μL
    Buffer
    MGMT Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Target
    MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
    Andere Bezeichnung
    MGMT (MGMT Produkte)
    Hintergrund

    Synonyms: Methylated-DNA--protein-cysteine methyltransferase, EC 2.1.1.63, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase

    Background: MGMT or O-6-methylguanine-DNA methyltransferase is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens. The methylation of the MGMT promoter in gliomas is a useful predictor of the responsiveness of the tumors to alkylating agents (1). The clinical trial substantiates the predictive value of O-6-methylguanine-DNA methyltransferase promoter methylation in glioblastoma patients treated with temozolomide (2). Methylation of the MGMT promoter in tumors is associated with longer survival in glioblastoma patients. MGMT Protein is ideal for investigators involved in Signaling Proteins, Acetyl/Methyltransferase Proteins, Apoptosis/Autophagy, Cancer, Cell Cycle, and Neurobiology research.

    Pathways
    DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus
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