PDGF Protein

Produktnummer ABIN6700388

Hersteller Produkt- Nr.: 009-001-w10-0010

Produktdetails

Target: PDGF

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Hersteller Produkt- Nr.: 009-001-w10-0010

Hersteller: Rockland

Verwendungszweck: Human Platelet Derived Growth Factor-AB Recombinant Protein

Aufreinigung: platelet Derived Growth Factor-AB purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Reinheit: 95,00%

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically less than 1 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Other: User Optimized

Application_Note: Platelet Derived Growth Factor-AB Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Platelet Derived Growth Factor-AB in immunological assays.

Kommentare:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Konzentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: PDGF

Andere Bezeichnung: PDGFA/PDGFB

Hintergrund:

Synonyms: PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, proto-oncogene c-Sis

Background: Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF. Recombinant human PDGF-AB is a non-glycosylated, disulfide-linked heterodimer, containing one 14.3 kDa alpha-chain and one 12.1 kDa beta-chain, with total molecular weight of 26.4 kDa.

UniProt: P04085