PDGFB Protein

Produktnummer ABIN6700408

Hersteller Produkt- Nr.: 010-001-w12-0010

Produktdetails

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Hersteller Produkt- Nr.: 010-001-w12-0010

Hersteller: Rockland

Verwendungszweck: Mouse Platelet Derived Growth Factor BB Recombinant Protein

Aufreinigung: platelet Derived Growth Factor BB purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Reinheit: 98,00%

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is measured by the dose-dependent proliferation in mouse 3T3 fibroblasts and is typically 1-2 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Other: User Optimized

Application_Note: Platelet Derived Growth Factor-BB Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Platelet Derived Growth Factor-BB in immunological assays.

Kommentare:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Konzentration: 0.1 mg/mL

Buffer:

Buffer: 0.01 M Sodium Citrate, pH 3.0

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Andere Bezeichnung: Pdgfb (PDGFB Produkte)

Hintergrund:

Synonyms: PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis

Background: Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF. Recombinant mouse PDGF-BB is a non-glycosylated, disulfide-linked homodimer, containing two 109 amino acids chains, with a total molecular weight of 24.6 kDa.

UniProt: P31240

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling