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Retinoblastoma 1 Protein (RB1) (GST tag)

Rockland 009-001-t16s RB1 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >85% WB
Rockland
Produktnummer ABIN6700477
Hersteller Produkt- Nr.: 009-001-t16s
  • Target Alle Retinoblastoma 1 (RB1) Proteine anzeigen
    Retinoblastoma 1 (RB1)
    Protein-Typ
    Recombinant
    Spezies
    • 5
    • 1
    Human
    Quelle
    • 4
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses Retinoblastoma 1 Protein ist gelabelt mit GST tag.
    Applikation
    Western Blotting (WB)
    Hersteller Produkt- Nr.
    009-001-t16s
    Hersteller
    Rockland
    Verwendungszweck
    Rb recombinant protein-GST fusion protein
    Aufreinigung
    Recombinant human Rb (773-928) was expressed in E.coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.
    Reinheit
    >85%
    Top Product
    Discover our top product RB1 Protein
  • Applikationshinweise

    Western_Blot_Dilution: User Optimized

    Other: Kinase Assay-User Optimized

    Application_Note: Rb Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~44 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.2 μg/μL
    Buffer
    Rb Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Target
    Retinoblastoma 1 (RB1)
    Andere Bezeichnung
    RB1 (RB1 Produkte)
    Hintergrund

    Synonyms: RB1, OSRC, pRb, pp110, p105-Rb, Retinoblastoma 1

    Background: Rb is the retinoblastoma gene product and was the first tumor suppressor cloned. Rb is a negative regulator of the cell cycle through its ability to bind the transcription factor E2F and repress transcription of genes required for S phase (1). Rb can bind to MDM2 and this overcomes both the antiapoptotic function of MDM2 and the MDM2-dependent degradation of p53. Rb associates with the Polycomb group (PcG) proteins to form a repressor complex that blocks entry of cells into mitosis (2). Rb colocalizes with nuclear PcG complexes and is important for association of PcG complexes with nuclear targets. Rb Protein is ideal for investigators involved in Signaling Reagents, Protein Substrates, Cancer, Cell Cycle, and Neurobiology research.

    NCBI Accession
    NM_000321
    Pathways
    Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagie
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