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SCYL3 Protein (GST tag)

Rockland 009-001-t27s SCYL3 Spezies: Human Wirt: Insect cells (Sf9) Recombinant >85% WB
Rockland
Produktnummer ABIN6700491
Hersteller Produkt- Nr.: 009-001-t27s
  • Target Alle SCYL3 Proteine anzeigen
    SCYL3 (SCY1-Like 3 (SCYL3))
    Protein-Typ
    Recombinant
    Spezies
    Human
    Quelle
    • 1
    • 1
    Insect cells (Sf9)
    Aufreinigungstag / Konjugat
    Dieses SCYL3 Protein ist gelabelt mit GST tag.
    Applikation
    Western Blotting (WB)
    Hersteller Produkt- Nr.
    009-001-t27s
    Hersteller
    Rockland
    Verwendungszweck
    SCYL3 recombinant protein-GST fusion protein
    Aufreinigung
    Recombinant human SCYL3 (2-end) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.
    Reinheit
    >85%
    Top Product
    Discover our top product SCYL3 Protein
  • Applikationshinweise

    Western_Blot_Dilution: User Optimized

    Application_Note: SCYL3 Protein is suitable for use in Western Blot. Expect a band approximately ~110 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.1 μg/μL
    Buffer
    SCYL3 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Target
    SCYL3 (SCY1-Like 3 (SCYL3))
    Andere Bezeichnung
    SCYL3 (SCYL3 Produkte)
    Hintergrund

    Synonyms: PACE-1, PACE1, RP1-97P20.2, Protein-associating with the carboxyl-terminal domain of ezrin, Ezrin-binding protein PACE-1, SCY1-like protein 3, SCYL3

    Background: SCYL3 encodes a protein with a kinase domain and four HEAT repeats which interact with the C-terminal domain of ezrin, an ERM protein which play a role in cell adhesion and migration (1). C-terminal domain of SCYL3 interacts with the C-terminal alpha-helical domain of ezrin. Biochemical assays indicate that SCYL3 had associated rather than intrinsic protein kinase activity. SCYL3 is associated with the cytoplasmic face of the Golgi apparatus. This distribution is dependent upon the presence of the SCYL3 N-terminal myristoylation consensus sequence but is not dependent on an association with ezrin. SCYL3 is highly expressed in breast carcinoma cells and adult colon. SCYL3 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Inflammation, Invasion/Metastasis, and Neurobiology research.

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