SIRT2 Protein (GST tag)

Produktnummer ABIN6700529

Hersteller Produkt- Nr.: 009-001-t32s

Produktdetails

Target: SIRT2 (Sirtuin 2 (SIRT2))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses SIRT2 Protein ist gelabelt mit GST tag.

Applikation: Western Blotting (WB)

Hersteller Produkt- Nr.: 009-001-t32s

Hersteller: Rockland

Verwendungszweck: SIRT2 recombinant protein-GST fusion protein

Aufreinigung: Full-length recombinant human SIRT2 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.

Reinheit: >85%

Anwendungsinformationen

Applikationshinweise:

Western_Blot_Dilution: User Optimized

Application_Note: SIRT2 Protein is suitable for use in Western Blot. Expect a band approximately ~64 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 μg/μL

Buffer: SIRT2 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: SIRT2 (Sirtuin 2 (SIRT2))

Andere Bezeichnung: SIRT2 (SIRT2 Produkte)

Hintergrund:

Synonyms: Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIRT2, SIR2L, SIR2L2, Sirtuin 2

Background: SIRT2 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT2 being a member of class I. Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington's disease (1). Huntington's disease is characterized by increased sterols synthesis in neuronal cells and this process is reversed by SIRT2 inhibition. SIRT2 can deacetylate lys40 of alpha-tubulin both in vitro and in vivo (2). Knockdown of SIRT2 via small interfering RNA results in tubulin hyperacetylation. SIRT2 Protein is ideal for investigators involved in Signaling Proteins, Deacetylase/Demethylase Proteins, Cancer, Cell Cycle, and Inflammation research.

NCBI Accession: NM_030593