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Sonic Hedgehog Protein (SHH)

Rockland 009-001-w24-0005 SHH Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant 97,00% SDS
Rockland
Produktnummer ABIN6700556
Hersteller Produkt- Nr.: 009-001-w24-0005
  • Target Alle Sonic Hedgehog (SHH) Proteine anzeigen
    Sonic Hedgehog (SHH)
    Protein-Typ
    Recombinant
    Spezies
    • 14
    • 11
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 20
    • 18
    • 5
    • 1
    • 1
    Escherichia coli (E. coli)
    Applikation
    SDS-PAGE (SDS)
    Hersteller Produkt- Nr.
    009-001-w24-0005
    Hersteller
    Rockland
    Verwendungszweck
    Human Sonic Hedgehog Recombinant Protein
    Aufreinigung
    Sonic Hedgehog purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
    Reinheit
    97,00%
    Endotoxin-Niveau
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    The activity is measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 (CCL-226) fibroblasts and is typically 0.8-1 μg/mL.
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  • Applikationshinweise

    Other: User Optimized

    Application_Note: Sonic Hedgehog Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Sonic Hedgehog in immunological assays.

    Kommentare

    Suggested_Applications: Cellular Assay

    Other_Performance_Data:

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 5 μL (5-50 μL)

    Buffer

    Buffer: 0.01 M Sodium Phosphate, pH 7.5

    Stabilizer: None

    Konservierungsmittel
    Without preservative
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Haltbarkeit
    6 months
  • Target
    Sonic Hedgehog (SHH)
    Andere Bezeichnung
    SHH (SHH Produkte)
    Hintergrund

    Synonyms: HHG-1

    Background: Sonic hedgehog (SHH) is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60 % homology and all signal via the same receptors. Recombinant human SHH is a non-glycosylated protein, containing 175 amino acids, with a molecular weight of 19.7 kDa. The Cys at position 25 has been substituted with Ile.

    UniProt
    Q15465
    Pathways
    Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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