MAPT Protein (Pro301Ser-Mutant)

Produktnummer ABIN6700659

Hersteller Produkt- Nr.: 009-001-u01s

Produktdetails

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Protein-Typ: Recombinant

Proteineigenschaft: Pro301Ser-Mutant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB)

Hersteller Produkt- Nr.: 009-001-u01s

Hersteller: Rockland

Verwendungszweck: Tau-441 (P301S) recombinant protein

Aufreinigung: Recombinant human tag-free Tau-441 (P301S) was expressed in E. coli cells. The purity was determined to be >90% by densitometry.

Reinheit: >90%

Anwendungsinformationen

Applikationshinweise:

Western_Blot_Dilution: User Optimized

Other: Kinase Assay-User Optimized

Application_Note: Tau-441 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~63 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Kommentare:

EM

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 μg/μL

Buffer: Tau-441 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen

Flach, Leu, Martinisi, Skachokova, Frank, Tolnay, Stahlberg, Winkler: "Trans-seeding of Alzheimer-related tau protein by a yeast prion." in: Alzheimer's & dementia : the journal of the Alzheimer's Association, Vol. 18, Issue 12, pp. 2481-2492, (2023) (PubMed).

Antigendetails

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Andere Bezeichnung: MAPT (MAPT Produkte)

Hintergrund:

Synonyms: Tau-F, (N2R4), Tau-4, MAPT, MSTD, PPND, DDPAC, MAPTL, MTBT1, MTBT2, FTDP-17, FLJ31424, MGC138549, Microtubule-associated protein tau

Background: Tau-441 or Tau-F is a member of the Tau family of proteins which function to stabilize the microtubules by binding to them. Tau proteins are subject to phosphorylation and this phenomenon regulates the association of the Tau protein with the microtubules (1). Deposits of Alzheimer's disease AD-associated proteins, such as hyperphosphorylated Tau, as well as other shared misfolded proteins, such as, β-amyloid precursor protein (βAPP), ubiquitin, and various chaperones and protein kinases are thought to play a pathologic role in the cognitive decline and muscular failure. Malfunctioning of Tau proteins is associated with microtubules disintegration and collapsing of the neuronal transport system (2). Tau-441 Protein is ideal for investigators involved in Signaling Proteins, Tau Proteins, Invasion/Metastasis, Neurobiology, and p38 Pathway research.

UniProt: P10636-8

Pathways: MAPK Signalweg, Microtubule Dynamics, M Phase, Regulation of Cell Size