Thrombopoietin Protein (THPO)

Produktnummer ABIN6700706

Hersteller Produkt- Nr.: 010-001-w32-0100

Produktdetails

Target: Thrombopoietin (THPO)

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Hersteller Produkt- Nr.: 010-001-w32-0100

Hersteller: Rockland

Verwendungszweck: Mouse Thrombopoietin Recombinant Protein

Aufreinigung: Thrombopoietin purity was determined to be greater than 97% as determined by reducing and non-reducing SDS-pAGE.

Reinheit: 97,00%

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent stimulation of MO7e cells and is typically less than 1 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Other: User Optimized

Application_Note: Thrombopoietin Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Thrombopoietin in immunological assays.

Kommentare:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Konzentration: 0.1 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: Thrombopoietin (THPO)

Andere Bezeichnung: Thpo (THPO Produkte)

Hintergrund:

Synonyms: C-mpl ligand (ML), Megakaryocyte Colony Stimulating Factor, Megakaryocyte growth and development factor (MGDF), Myeloproliferative leukemia virus oncogene ligan, c-MPL ligand

Background: Thrombopoietin (TPO) is a growth factor that is produced by the liver and kidney. TPO acts through the TPO receptor to promote megakaryocyte maturation and differentiation, which leads to the production of platelets. Recombinant mouse TPO is a non-glycosylated protein, containing 174 amino acids (which comprise the receptor binding domain), with a molecular weight of 18.7 kDa.

UniProt: P40226

Pathways: JAK-STAT Signalweg, Hormone Activity