Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

VEGFA Protein

Rockland 012-001-b99-0010 VEGFA Spezies: Ratte Wirt: Escherichia coli (E. coli) Recombinant 97,00% SDS
Rockland
Produktnummer ABIN6700875
Hersteller Produkt- Nr.: 012-001-b99-0010
  • Target Alle VEGFA Proteine anzeigen
    VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
    Protein-Typ
    Recombinant
    Spezies
    • 21
    • 21
    • 14
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte
    Quelle
    • 46
    • 14
    • 13
    • 5
    • 1
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Applikation
    SDS-PAGE (SDS)
    Hersteller Produkt- Nr.
    012-001-b99-0010
    Hersteller
    Rockland
    Verwendungszweck
    Rat Vascular Endothelial Growth Factor-165 Recombinant Protein
    Aufreinigung
    Vascular Endothelial Growth Factor-165 purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
    Reinheit
    97,00%
    Endotoxin-Niveau
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    The activity is determined by the dose-dependent induced proliferation of Human umbilical vein endothelial cells (HUVECs) and is typically 4.0-8.0 ng/mL.
    Top Product
    Discover our top product VEGFA Protein
  • Applikationshinweise

    Other: User Optimized

    Application_Note: Endothelial Growth Factor-165 Recombinant Protein has been tested by SDS-PAGE and bioactivity and is suitable as a control for polyclonal or monoclonal anti-Endothelial Growth Factor-165 in immunological assays.

    Kommentare

    Suggested_Applications: Cellular Assay

    Other_Performance_Data:

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 10 μL (10-100 μL)

    Buffer

    Buffer: 0.01 M Sodium Phosphate, pH 7.5

    Stabilizer: None

    Konservierungsmittel
    Without preservative
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Haltbarkeit
    6 months
  • Target
    VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
    Andere Bezeichnung
    Vegfa (VEGFA Produkte)
    Hintergrund

    Synonyms: VEGF-A, glioma-derived endothelial cell mitogen, Vascular permeability factor (VPF)

    Background: Vascular Endothelial Growth Factor-A (VEGF-A) was originally isolated from tumor cells and is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, VEGF-A may play a role in stimulating vasodilatation via nitric oxide-dependent pathways. VEGF-A has several variants, VEGF-165 being the most abundant. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 % . Recombinant rat VEGF-165 is a disulfide linked homodimer, containing two 165 amino acids chains, with a total molecular weight of 38.8 kDa.

    UniProt
    P16612-2
    Pathways
    RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
Kundenservice