IL-17A/F Protein

Produktnummer ABIN6700928

Hersteller Produkt- Nr.: 009-001-v56-0005

Produktdetails

Target: IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Hersteller Produkt- Nr.: 009-001-v56-0005

Hersteller: Rockland

Verwendungszweck: Human Interleukin-17AF Heterodimer Recombinant Protein

Aufreinigung: Interleukin-17AF Heterodimer purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Reinheit: 98,00%

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by a dose-dependent production of IL-6 in cultured mouse NIH 3T3 fibroblasts and is typically 3-15 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Other: User Optimized

Application_Note: Interleukin-17AF Heterodimer Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-17AF Heterodimer in immunological assays.

Kommentare:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))

Andere Bezeichnung: IL17A/IL17F (IL-17A/F Produkte)

Hintergrund:

Synonyms: IL17 heterodimer, IL17AF heterodimer, CTLA-8 ML-1 dimer, Interleukin 17AF, Interleukin-17AF heterodimer

Background: Interleukin-17AF (IL-17AF) is a member of the IL-17 family of proteins produced by a subset of T cells, called Th17, following stimulation with IL-23. Since IL-17AF is thought to signal through the IL-17RA receptor, its biological function is similar to that of IL-17A in that it induces the production of a variety of chemokines, in addition to airway neutrophilia. In regard to these functions, IL-17AF has less activity than the IL-17A homodimer but, greater activity than the IL-17F homodimer. Human and rat IL-17AF both show activity on mouse cells. Recombinant human IL-17AF is a non-glycosylated heterodimer, containing one IL-17A subunit and one IL-17F subunit. The dimer has a total of 271 amino acids, with an approximate molecular weight of 30.7 kDa.

UniProt: Q16552