tau Protein (full length, P301S)

Produktnummer ABIN6929397

Recombinant tau-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für tau Protein (full length, P301S) (ABIN6929397)

Target: tau (tau Protein)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Western Blotting (WB), In vitro Assay (in vitro), In vivo Studies (in vivo)

Produktdetails

Proteineigenschaft: P301S, full length

Verwendungszweck: Active Human Recombinant Tau441 (2N4R), P301S mutant Protein Pre-formed Fibrils

Sequenz: MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKESPLQT PTEDGSEEPG SETSDAKSTP TAEDVTAPLV DEGAPGKQAA AQPHTEIPEG TTAEEAGIGD TPSLEDEAAG HVTQARMVSK SKDGTGSDDK KAKGADGKTK IATPRGAAPP GQKGQANATR IPAKTPPAPK TPPSSGEPPK SGDRSGYSSP GSPGTPGSRS RTPSLPTPPT REPKKVAVVR TPPKSPSSAK SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHV SGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE NAKAKTDHGA EIVYKSPVVS GDTSPRHLSN VSSTGSIDMV DSPQLATLAD EVSASLAKQG L

Spezifität: ~45.8 kDa

Aufreinigung: Ion-exchange Purified

Biological Activity Comment: Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when active tau PFFs are combined with active tau monomers.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: Lot specific

Buffer: 10 mM HEPES, 100 mM NaCl pH 7.4

Lagerung: -80 °C

Antigendetails

Target: tau (tau Protein)

Andere Bezeichnung: Tau

Hintergrund: Alzheimer's Disease (AD) is the most common neurodegenerative disease, affecting 10 % of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). 2N4R, or Tau-441 is the full length tau protein. P301S is a mutation encoded by exon 10 (4) that impairs the ability of tau to assemble microtubules (5).

NCBI Accession: NP_005901

UniProt: P10636