tau Protein (partial, Pro301Leu-Mutant)

Produktnummer ABIN6929400

Produktdetails

Target: tau (tau Protein)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: Pro301Leu-Mutant, partial

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Western Blotting (WB), In vitro Assay (in vitro), In vivo Studies (in vivo)

Verwendungszweck: Active Human Recombinant Tau (K18), P301L mutant Protein Pre-formed Fibrils

Sequenz: SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHV LGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE

Spezifität: ~15.1 kDa

Aufreinigung: Ion-exchange Purified

Biological Activity Comment: Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when active tau PFFs are combined with active tau monomers.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: Lot specific

Buffer: 10 mM HEPES, 100 mM NaCl pH 7.4

Lagerung: -80 °C

Antigendetails

Target: tau (tau Protein)

Andere Bezeichnung: Tau (tau Produkte)

Synonyme: DDPAC Protein, FTDP-17 Protein, MAPTL Protein, MSTD Protein, MTBT1 Protein, MTBT2 Protein, PPND Protein, TAU Protein, tau Protein, PHF-tau Protein, microtubule associated protein tau Protein, MAPT Protein

Hintergrund: Alzheimer's Disease (AD) is the most common neurodegenerative disease, affecting 10 % of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). K18 is a truncated form of human tau containing only the 4 microtubule binding repeats (5). P301L (PL) is a mutation where proline is replaced by leucine at codon 301 of tau, and has been linked to frontotemporal dementia (6).

UniProt: P10636