Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Arg683) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with a 6xHis tag at the C-terminus.
Reinheit
> 90 % by SDS-PAGE.
Sterilität
0.22 μm filtered
Endotoxin-Niveau
< 0.1 EU/μg of the protein by LAL method.
Biological Activity Comment
1.Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 Spike S1 at 2 μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.03-7.12 ng/mL.< /br>2.Immobilized Human ACE2 on COOH Chip can bind SARS-COV-2 Spike S1 with an affinity constant of 27.3 nM as determined in a SPR assay (Nicoya OpenSPR).
Spezies: SARS Coronavirus-2 (SARS-CoV-2)
Wirt: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Konzentration
1 mg/mL
Buffer
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.
Handhabung
Avoid repeated freeze-thaw cycles.
Lagerung
4 °C,-20 °C,-80 °C
Informationen zur Lagerung
Store the lyophilized protein at -20°C to -80 °C for long term.After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.|or This product is stable at ≤ -70°C for up to 6 months from the date of receipt. |For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.