SPAM1 Protein (AA 36-482) (Fc Tag)

Produktnummer ABIN6964321

Produktdetails

Target: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 36-482

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SPAM1 Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Recombinant human SPAM1 protein with C-terminal human Fc tag

Spezifität: SPAM1 (Leu36-Tyr482) hFc (Glu99-Ala330)

Produktmerkmale: Extracellular Domain Protein

Aufreinigung: Purified from cell culture supernatant by affinity chromatography

Reinheit: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Andere Bezeichnung: SPAM1 (SPAM1 Produkte)

Synonyme: AV039194 Protein, Ph-20 Protein, PH-20 Protein, PH20 Protein, HYA1 Protein, HYAL1 Protein, HYAL3 Protein, HYAL5 Protein, SPAG15 Protein, Spam Protein, XEH1 Protein, spam1 Protein, im:6902904 Protein, zgc:158668 Protein, sperm adhesion molecule 1 Protein, sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) L homeolog Protein, sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) Protein, Spam1 Protein, SPAM1 Protein, spam1.L Protein, spam1 Protein

Hintergrund: Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1, another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Molekulargewicht: predicted molecular mass of 77.2 kDa after removal of the signal peptide. The apparent molecular mass of SPAM1-hFc is 100-130 kDa due to glycosylation.

UniProt: P38567