ITGAV/ITGB8 Protein (His tag)

Produktnummer ABIN7121506

Produktdetails

Target: ITGAV/ITGB8 (Integrin alpha V beta 8 (ITGAV/ITGB8))

Protein-Typ: Recombinant

Spezies: Cynomolgus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses ITGAV/ITGB8 Protein ist gelabelt mit His tag.

Verwendungszweck: Cynomolgus Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein, His Tag&Tag Free

Sequenz: Phe 31 - Pro 993 (ITGAV) & Glu 43 - Ser 681 (ITGB8)

Produktmerkmale: Cynomolgus ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free (IT8-C52W9) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Pro 993 (ITGAV) & Glu 43 - Ser 681 (ITGB8) (Accession # A0A2K5WCD3-1 (ITGAV) & G7P0S0-1 (ITGB8).

Reinheit: >95 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: Cynomolgus Integrin alpha V beta 8 (ITGAV&ITGB6) Heterodimer Protein, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 113.1 kDa (ITGAV) and 76.0 kDa (ITGB8). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Glu 43 (ITGB8). The non-reducing (NR) protein migrates as kDa (ITGAV) & 75-85 kDa (ITGB8) respectively due to glycosylation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 50 mM Tris, 150 mM NaCl, pH 7.5

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Antigendetails

Target: ITGAV/ITGB8 (Integrin alpha V beta 8 (ITGAV/ITGB8))

Andere Bezeichnung: Integrin alpha V beta 8 (ITGAV&ITGB8) (ITGAV/ITGB8 Produkte)

Hintergrund: Synonyms: Integrin alpha V beta 8,ITGAV&ITGB8,
Description: Integrin alpha V beta 8 (ITGAV & ITGB8 or ITGAVB8) is expressed in yolk sac, placenta, brain perivascular astrocytes, Schwann cells, renal glomerular mesangial cells and pulmonary epithelial cells. Unlike other alpha V integrins, ITGAVB8 does not appear to assume different activation states, and the cytoplasmic tail does not connect to the cytoskeleton. It binds ligands containing an RGD motif, including vitronectin, fibrin and the latency associated peptide (LAP) of the latent TGF-beta complex. High affinity binding of alpha V beta 8 to LAP allows proteolytic cleavage by MT1-MMP, which releases active TGF-beta. This mechanism differs from that of alpha V beta 6, the other alpha V integrin which can activate TGF-beta from latency through non-proteolytic mechanisms. Downstream effects of TGF-beta activation include control of cell growth and associated vascularization.

Molekulargewicht: 113.1 kDa (ITGAV) & 76.0 kDa (ITGB8)