SPAM1 Protein
Kurzübersicht für SPAM1 Protein (ABIN7199566)
Target
Alle SPAM1 Proteine anzeigenProtein-Typ
Spezies
Quelle
Reinheit
Güteklasse
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Verwendungszweck
- Human PH20 / SPAM1 Protein, Tag Free (MALS verified)
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Sequenz
- Leu 36 - Ser 490
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Produktmerkmale
- Human PH20, Tag Free (PH0-H5219) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Ser 490 (Accession # P38567-1).
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Endotoxin-Niveau
- Less than 1.0 EU per μg by the LAL method.
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Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) (AA 346-445) protein (GST tag)
SPAM1 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) (AA 1-509) protein (GST tag)SPAM1 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) (AA 36-482) protein (Fc Tag)SPAM1 Spezies: Human Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SPAM1 Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) (AA 36-482) (Active) protein (His tag)SPAM1 Spezies: Human Wirt: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Applikationshinweise
- This protein carries no "tag". The protein has a calculated MW of 52.6 kDa. The protein migrates as 65-70 kDa under reducing (R) condition due to glycosylation.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Buffer
- 50 mM Tris, 100 mM NaCl, pH 7.5
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Lagerung
- -20 °C
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Informationen zur Lagerung
- -20°C
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- SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))
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Andere Bezeichnung
- / SPAM1
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Hintergrund
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Synonyms: SPAM1,PH-20,HYAL3,HYA1,HYAL1,HYAL5,SPAG15,
Description: Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N - acetyl - beta - D - glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. -
Molekulargewicht
- 52.6 kDa
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NCBI Accession
- NP_694859
Target
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