Dieses SARS-CoV-2 Spike Protein ist gelabelt mit His tag.
Verwendungszweck
SARS-CoV-2 Spike Trimer, His Tag (BA.2+L452M/Omicron) (MALS verified)
Sequenz
Val 16 - Pro 1213
Produktmerkmale
SARS-CoV-2 Spike Trimer, His Tag (BA.2+L452M/Omicron) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (T19I, LPP24-26del, A27S, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452M, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P). The spike mutations are identified in multiple SARS-CoV-2 Omicron variants (Pango lineage: BA.2.9.1 and BA.2.13, GISAID clade: GRA). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Spezies: SARS Coronavirus-2 (SARS-CoV-2)
Wirt: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Applikationshinweise
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138.1 kDa. The protein migrates as kDa under reducing (R) condition due to glycosylation.
E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
Hintergrund
Synonyms: Spike,S protein,Spike glycoprotein,S glycoprotein, It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.