Acetylcholinesterase Protein (AChE) (His tag)

Produktnummer ABIN7320239

Recombinant Acetylcholinesterase-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für Acetylcholinesterase Protein (AChE) (His tag) (ABIN7320239)

Target: Acetylcholinesterase (AChE)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: > 97 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses Acetylcholinesterase Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse Acetylcholinesterase/ACHE Protein (His Tag)

Sequenz: Met 1-Leu 614

Produktmerkmale: A DNA sequence encoding the mouse ACHE (NP_033729.1) (Met 1-Leu 614) was expressed, with a polyhistidine tag at the C-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave Acetylthiocholine. The specific activity is > 250 nmols/min/μg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: Acetylcholinesterase (AChE)

Andere Bezeichnung: Acetylcholinesterase/ACHE

Hintergrund: ME1a,mE1b,mE1c,mE1c-long,mE1d,mE1d',mE1e,Acetylcholinesterase, also known as ACHE, is an enzyme that degrades the neurotransmitter acetylcholine, producing choline and an acetate group. ACHE plays a crucial role in nerve impulse transmission at cholinergic synapses by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). ACHE appears to be a potential therapeutic target at muscle injuries including organophosphate myopathy. It is an externally oriented membrane-bound enzyme and its main physiological role is termination of chemical transmission at cholinergic synapses and secretory organs by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). ACHE plays important roles in the cholinergic system, and its dysregulation is involved in a variety of human diseases. ACHE was significantly down-regulated in the cancerous tissues of 69.2 % of hepatocellular carcinoma (HCC) patients, and the low ACHE expression in HCC was correlated with tumor aggressiveness, an elevated risk of postoperative recurrence, and a low survival rate. Both the recombinant ACHE protein and the enhanced expression of ACHE significantly inhibited HCC cell growth in vitro and tumorigenicity in vivo. ACHE as a tumor growth suppressor in regulating cell proliferation, the relevant signaling pathways, and the drug sensitivity of HCC cells. Thus, ACHE is a promising independent prognostic predictor for HCC recurrence and the survival of HCC patients. ACHE is inhibited by organophosphate and carbamate pesticides. However, this enzyme is only slightly inhibited by organophosphorothionates.

Molekulargewicht:

Calculated MW: 66.2 kDa

Observed MW: 66.2 kDa

NCBI Accession: NP_033729

UniProt: P21836

Pathways: Skeletal Muscle Fiber Development