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AGO2 Protein (His tag)

AGO2 Spezies: Maus Wirt: Baculovirus infected Insect Cells Recombinant > 88 % as determined by SDS-PAGE
Produktnummer ABIN7320195
  • Target Alle AGO2 Proteine anzeigen
    AGO2 (Argonaute 2 (AGO2))
    Protein-Typ
    Recombinant
    Spezies
    • 7
    • 1
    Maus
    Quelle
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Baculovirus infected Insect Cells
    Aufreinigungstag / Konjugat
    Dieses AGO2 Protein ist gelabelt mit His tag.
    Verwendungszweck
    Recombinant Mouse AGO2/Argonaute 2/EIF2C2 Protein (His Tag)
    Sequenz
    Met 1-Ala 860
    Produktmerkmale
    A DNA sequence encoding the mouse AGO2 (Q8CJG0) (Met 1-Ala 860) was fused with a polyhistidine tag at the N-terminus.
    Reinheit
    > 88 % as determined by SDS-PAGE
    Endotoxin-Niveau
    < 1.0 EU per μg of the protein as determined by the LAL method.
    Top Product
    Discover our top product AGO2 Protein
  • Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Please refer to the printed manual for detailed information.
    Buffer
    Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
    Lagerung
    4 °C,-20 °C,-80 °C
    Informationen zur Lagerung
    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Target
    AGO2 (Argonaute 2 (AGO2))
    Andere Bezeichnung
    AGO2/Argonaute 2/EIF2C2 (AGO2 Produkte)
    Synonyme
    EIF2C2 Protein, T19E23.7 Protein, T19E23_7 Protein, argonaute 2 Protein, Q10 Protein, Eif2c2 Protein, Argonaute2 Protein, eif2c1 Protein, eif2c2 Protein, 1110029L17Rik Protein, 2310051F07Rik Protein, AI225898 Protein, AL022874 Protein, AW546247 Protein, ENSMUSG00000072493 Protein, Gerp95 Protein, Gm10365 Protein, mKIAA4215 Protein, AGO2 Protein, AG02 Protein, AGO 2 Protein, Ago-2 Protein, Ago2 Protein, CG13452 Protein, CG7439 Protein, Dm Ago2 Protein, Dmel\\CG7439 Protein, ago Protein, ago-2 Protein, ago2 Protein, dAGO2 Protein, dAgo2 Protein, eIF2C2 Protein, argonaute 2, RISC catalytic component Protein, Argonaute family protein Protein, argonaute 2, RISC catalytic component L homeolog Protein, argonaute RISC catalytic subunit 2 Protein, argonaute RISC catalytic component 2 Protein, Argonaute 2 Protein, AGO2 Protein, Ago2 Protein, ago2.L Protein, ago2 Protein
    Hintergrund

    Background: Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.

    Synonym: 1110029L17Rik,2310051F07Rik,AI225898,AL022874,AW546247,Eif2c2,ENSMUSG00000072493,Gerp95,Gm10365,mKIAA4215

    Molekulargewicht
    99 kDa
    UniProt
    Q8CJG0
    Pathways
    Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Ribonucleoprotein Complex Subunit Organization
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