CMBL Protein (His tag)

Produktnummer ABIN7317566

Produktdetails

Target: CMBL (Carboxymethylenebutenolidase Homolog (CMBL))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses CMBL Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human CMBL Protein (His Tag)

Sequenz: Met 1-Met 245

Produktmerkmale: A DNA sequence encoding the human CMBL (Q96DG6) (Met 1-Met 245) was expressed, with a N-terminal polyhistidine tag.

Reinheit: > 97 % as determined by reducing SDS-PAGE.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 0.1 % Brij35, pH 8.0

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: CMBL (Carboxymethylenebutenolidase Homolog (CMBL))

Andere Bezeichnung: CMBL (CMBL Produkte)

Synonyme: JS-1 Protein, RGD1306952 Protein, 2310016A09Rik Protein, carboxymethylenebutenolidase homolog Protein, carboxymethylenebutenolidase-like (Pseudomonas) Protein, CMBL Protein, Cmbl Protein

Hintergrund:

Background: Carboxymethylenebutenolidase (CMBL), also known as 4-carboxymethylenebut-2-en-4-olide lactonohydrolase, maleylacetate enol- lactonase, dienelactone hydrolase, and carboxymethylene butenolide hydrolase, is a hydrolase specially belonging to the family of hydrolases. It maily acts on carboxylic ester bonds. CMBL is a human homolog of Pseudomonas dienelactone hydrolase involved in the bacterial halocatechol degradation pathway. The ubiquitous expression of human CMBL gene transcript in various tissues was observed. CMBL was demonstrated to be the primary olmesartan medoxomil (OM) bioactivating enzyme in the liver and intestine. The recombinant human CMBL expressed in mammalian cells was clearly shown to activate OM. The recombinant CMBL also converted other prodrugs having the same ester structure as OM, faropenem medoxomil and lenampicillin, to their active metabolites. CMBL exhibited a unique sensitivity to chemical inhibitors, thus, being distinguishable from other known esterases.  

Synonym: JS-1

Molekulargewicht: 30 kDa

UniProt: Q96DG6