Elastase 4 Protein (His tag)

Produktnummer ABIN7195125

Produktdetails

Target: Elastase 4 (CTRC) (Chymotrypsin C (Caldecrin) (CTRC))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Elastase 4 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Chymotrypsin C Protein (His Tag)(Active)

Sequenz: Met 1-Leu 268

Produktmerkmale: A DNA sequence encoding the human CTRC (Q99895) (Met 1-Leu 268) was fused with a polyhistidine tag at the C-terminus.

Reinheit: > 97 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate, SUC-Ala-Ala-Pro-Phe-AMC.The specific activity is >300 pmol/min/μg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: Elastase 4 (CTRC) (Chymotrypsin C (Caldecrin) (CTRC))

Andere Bezeichnung: Chymotrypsin C (CTRC Produkte)

Synonyme: CLCR Protein, ELA4 Protein, 1810044E12Rik Protein, bPTLP Protein, chymotrypsin C Protein, chymotrypsin-C Protein, chymotrypsin C (caldecrin) Protein, CTRC Protein, LOC478220 Protein, Ctrc Protein

Hintergrund:

Background: Chymotrypsin C (abbreviated for CTRC); also known as caldecrin or elastase4; is a digestive enzyme of the peptidase S1 family. This enzyme is synthesized as an inactivate chymotrypsinogen. On cleavage by trypsin into two parts that activate each other by removing two small peptides in a trans-proteolysis; chymotrypsin C produced. N-linked glycosylation of human CTRC is required for efficient folding and secretion; however; the N-linked glycan is unimportant for enzyme activity or inhibitor binding. It has been proposed that CTRC is a key regulator of digestive zymogen activation and a physiological co-activator of digestive carboxypeptidases proCPA1 and proCPA2. Mutations that abolish activity or secretion of CTRC increase the risk for chronic pancreatitis. It's speculated that CTRC might regulate pancreatic cancer cell migration in relation to cytokeratin 18 expression. The pancreatic cancer cell migration ability was downregulated in pancreatic cancer Aspc-1 cells that overexpressed CTRC; whereas the cell migration ability was upregulated in Aspc-1 cells in which CTRC was suppressed. 

Synonym: Chymotrypsin-C; Caldecrin; CTRC; CLCR

Molekulargewicht: 29.3 kDa

UniProt: Q99895