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Complement C2 Protein (His tag)

Spezies: Human Wirt: HEK-293 Cells Recombinant > 97 % as determined by reducing SDS-PAGE. Active
Produktnummer ABIN7195225
  • Target Alle Complement C2 Proteine anzeigen
    Complement C2
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Spezies
    • 5
    • 2
    • 1
    Human
    Quelle
    • 4
    • 2
    • 2
    HEK-293 Cells
    Aufreinigungstag / Konjugat
    Dieses Complement C2 Protein ist gelabelt mit His tag.
    Verwendungszweck
    Recombinant Human C2/Complement Component 2 Protein (His Tag)(Active)
    Sequenz
    Met 1-Leu 752
    Produktmerkmale
    A DNA sequence encoding the human complement component 2 (C2) precursor (NP_000054.2) (Met 1-Leu 752) was expressed with a C-terminal polyhistidine tag.
    Reinheit
    > 97 % as determined by reducing SDS-PAGE.
    Endotoxin-Niveau
    < 1.0 EU per μg as determined by the LAL method.
    Biological Activity Comment
    Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5'Dithiobis (2-nitrobenzoic acid) (DTNB). The specific activity is >100 pmoles/min/μg.
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    Discover our top product Complement C2 Protein
  • Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Please refer to the printed manual for detailed information.
    Buffer
    Lyophilized from sterile PBS, pH 7.4
    Lagerung
    4 °C,-20 °C,-80 °C
    Informationen zur Lagerung
    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Target
    Complement C2
    Andere Bezeichnung
    C2/Complement Component 2 (Complement C2 Produkte)
    Synonyme
    CO2 Protein, complement C2 Protein, complement component 2 (within H-2S) Protein, C2 Protein
    Hintergrund

    Background: Complement component C2 is part of the classical complement pathway which plays a major role in innate immunity against infection. C2 is a glycoprotein synthesized in liver hepatocytes and several other cell types in extrahepatic tissues. This pathway is triggered by a multimolecular complex C1, and subsequently the single-chain form of C2 is cleaved into two chains referred to C2a and C2b by activated C1. The second component of complement (C2) is a multi-domain serine protease that provides catalytic activity for the C3 and C5 convertases of the classical and lectin pathways of human complement. C4b and C2 was investigated by surface plasmon resonance. C2a containing a serine protease domain combines with complement component C4b to form the C3 convertase C4b2a which is responsible for C3 activation, and leads to the stimulation of adaptive immune responses via Lectin pathway. C2 bound to C4b is cleaved by classical (C1s) or lectin (MASP2) proteases to produce C4bC2a. C2 has the same serine protease domain as C4bC2a but in an inactive zymogen-like conformation, requiring cofactor-induced conformational change for activity. Deficiency of C2 (C2D) is the most common genetic deficiency of the complement system, and two types of C2D have been recognized in the context of specific MHC haplotypes. C2D in human is reported to increase susceptibility to infection, and is associated with certain autoimmune diseases, such as rheumatological disorders.

    Synonym: ARMD14,CO2

    Molekulargewicht
    82.5 kDa
    NCBI Accession
    NP_000054
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