GFRA1 Protein (His tag)
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- Target Alle GFRA1 Proteine anzeigen
- GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
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Spezies
- Maus
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses GFRA1 Protein ist gelabelt mit His tag.
- Verwendungszweck
- Recombinant Mouse GFRA1/GDNFRA Protein (His Tag)(Active)
- Sequenz
- Met 1-Ser 425
- Produktmerkmale
- A DNA sequence encoding the extracellular domain (Met 1-Ser 425) of mouse GFRα1 isoform beta (AAB86600.1) was expressed with a C-terminal polyhistidine tag.
- Reinheit
- > 98 % as determined by SDS-PAGE
- Endotoxin-Niveau
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- 1. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.3-1.5 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF.2. Measured by its ability to bind with human GDNF in a functional ELISA.
- Top Product
- Discover our top product GFRA1 Protein
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))
- Andere Bezeichnung
- GFRA1/GDNFRA (GFRA1 Produkte)
- Synonyme
- gdnfr Protein, ret1l Protein, retl1 Protein, trnr1 Protein, gdnfra Protein, MGC122616 Protein, gfr-alpha-1 Protein, GDNFR Protein, GDNFRA Protein, GFR-ALPHA-1 Protein, RET1L Protein, RETL1 Protein, TRNR1 Protein, GDNFRALPHA Protein, GFRA1 Protein, AU042498 Protein, gfralpha1a Protein, zgc:110129 Protein, GDNF family receptor alpha 1 Protein, glial cell line derived neurotrophic factor family receptor alpha 1 Protein, gdnf family receptor alpha 1a Protein, gfra1 Protein, GFRA1 Protein, Gfra1 Protein, gfra1a Protein
- Hintergrund
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Background: Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 1 (GFRA1) is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA1 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. GDNF, a distantly related member of the transforming growth factor-β (TGF-â) superfamily, and its receptor components: GFRA1, Ret and neural cell adhesion molecule (NCAM) have been recently reported to be expressed in the testis and to be involved in the proliferation regulation of immature Sertoli cells.
Synonym: AU042498
- Molekulargewicht
- 46.3 kDa
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