IL-22 Protein

Produktnummer ABIN7320589

Recombinant IL-22-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für IL-22 Protein (ABIN7320589)

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by SDS-PAGE

Produktdetails

Verwendungszweck: Recombinant Mouse Interleukin-22/IL-22 Protein (Active)

Sequenz: Leu34-Val179

Produktmerkmale: Recombinant Mouse Interleukin-22 is produced by our E.coli expression system and the target gene encoding Leu34-Val179 is expressed.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. The ED50 for this effect is 281.45 pg/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.0.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Andere Bezeichnung: Interleukin-22/IL-22

Hintergrund:

Background: Interleukin-22 (IL-22) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid residue protein with a putative 33 amino acid signal peptide that is cleaved to generate a 147 amino acid mature protein that shares approximately 79 % and 22 % sequence identity with human IL22 and IL10, respectively. IL22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and up-regulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10Rβ (previously known as CRF2-4), belonging to the class II cytokine receptor family.

Synonym: Interleukin-22, IL-22, IL-10-Related T-Cell-Derived-Inducible Factor, IL-TIF, IL-TIF Alpha, Interleukin-22a, IL-22a, Il22, Il22a, Iltif, Iltifa

Molekulargewicht: 16.7 kDa

UniProt: Q9JJY9