CD130/gp130 Protein (His tag,Fc Tag)

Produktnummer ABIN7317708

Produktdetails

Target: CD130/gp130 (IL6ST) (Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CD130/gp130 Protein ist gelabelt mit His tag,Fc Tag.

Verwendungszweck: Recombinant Human IL6ST/CD130 Protein (His & Fc Tag)(Active)

Sequenz: Met 1-Ile 618

Produktmerkmale: A DNA sequence encoding the extracellular domain (Met 1-Ile 618) of human IL6ST (NP_002175.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: 1. Measured by its ability to bind human IL11Ra in a functional ELISA.2. Measured by its ability to inhibit the IL6 Rα enhancement of IL6 activity on M1 mouse myeloid leukemia cells (Saito, T. et al. 1991, J. Immunol. 147:168.). The ED50 for this effect is typically 0.05-0.15 μg/ml in the presence of 50 ng/ml recombinant human IL6sR and 100 ng/ml recombinant human IL6.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: CD130/gp130 (IL6ST) (Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST))

Andere Bezeichnung: IL6ST/CD130 (IL6ST Produkte)

Synonyme: CD130 Protein, CDW130 Protein, GP130 Protein, IL-6RB Protein, 5133400A03Rik Protein, AA389424 Protein, BB405851 Protein, D13Ertd699e Protein, gp130 Protein, Ac1055 Protein, Gp130 Protein, Il-6rb Protein, IL-6 Protein, interleukin 6 signal transducer Protein, IL6ST Protein, Il6st Protein, il6st Protein

Hintergrund:

Background: Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of all cytokine receptors. CD130/gp130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD130/gp130 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD130/gp130 plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. CD130/gp130 binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. CD130/gp130 may have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events.

Synonym: CD130,CDW130,GP130,IL-6RB

Molekulargewicht: 96 kDa

NCBI Accession: NP_002175

Pathways: JAK-STAT Signalweg, Cellular Glucan Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Cancer Immune Checkpoints