Nicastrin Protein (NCSTN) (His tag)

Produktnummer ABIN7197196

Produktdetails

Target: Nicastrin (NCSTN)

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Nicastrin Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Nicastrin/NCSTN Protein (His Tag)

Sequenz: Met 1-Glu 669

Produktmerkmale: A DNA sequence encoding the human NCSTN (NP_056146.1) extracellular domain (Met 1-Glu 669) was expressed, with a polyhistidine tag at the C-terminus.

Reinheit: > 93 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: Nicastrin (NCSTN)

Andere Bezeichnung: Nicastrin/NCSTN (NCSTN Produkte)

Synonyme: CG7012 Protein, Dmel\\CG7012 Protein, Nct Protein, Nic Protein, S5A Protein, ST96B Protein, agro Protein, dNct Protein, nic Protein, NCSTN Protein, DKFZp469O2418 Protein, APH2 Protein, 9430068N19Rik Protein, AA727311 Protein, Aph2 Protein, D1Dau13e Protein, Kiaa0253 Protein, mKIAA0253 Protein, nicastrin Protein, Nicastrin Protein, NCSTN Protein, nct Protein, ncstn Protein, CpipJ_CPIJ001617 Protein, Ncstn Protein, aph-2 Protein

Hintergrund:

Background: Nicastrin (NCST, or NCT), a single-pass membrane glycoprotein that harbors a large extracellular domain, is an essential component of the gamma-secretase complex. Several lines of evidence indicate that the members of these complexes could also contribute to the control of cell death. NCT controls cell death via phosphoinositide 3-kinase/Akt and p53-dependent pathways and that this function remains independent of the activity and molecular integrity of the gamma-secretase complexes. Increasing evidences have shown that Nicastrin/NCSTN plays a crucial role in gamma-cleavage of the amyloid precursor protein (APP). The glycoprotein Nicastrin is an essential component of the gamma-secretase complex, a high molecular weight complex which also contains the presenilin proteins, Aph-1 and Pen-2. The gamma-secretase complex is not only involved in APP processing but also in the processing of an increasing number of other type I integral membrane proteins. As the largest subunit of the gamma-secretase complex, Nicastrin plays a crucial role in its activation. Inhibition of NCSTN demonstrated an altered gamma-cleavage activity, suggesting its potential implication in developing Alzheimer's disease (AD). In addition, Nicastrin can function to maintain epithelial to mesenchymal transition during breast cancer progression. Anti-nicastrin polyclonal and monoclonal antibodies were able to decrease notch1 and vimentin expression and reduced the invasive capacity of breast cancer cells in vitro.

Synonym: APH2,ATAG1874,KIAA0253,NCSTN,RP11-517F10.1

Molekulargewicht: 72.4 kDa

NCBI Accession: NP_056146

Pathways: Notch Signalweg, Neurotrophin Signalübertragung