PDGF-BB Homodimer (Active) protein (His tag)

Produktnummer ABIN7197388

Produktdetails

Target: PDGF-BB Homodimer

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Hefe

Aufreinigungstag / Konjugat: His tag

Verwendungszweck: Recombinant Human PDGF-BB Protein (His Tag)(Active)

Sequenz: Ser82-Thr190

Produktmerkmale: A DNA sequence encoding the human PDGFB (NP_148937.1) (Ser82-Thr190) was expressed with a polyhistidine tag at the N-terminus.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Measured by its binding ability in a functional ELISA.2.Immobilized human PDGFB at 10μg/mL (100μL/well) can bind human PDGFRβ-Fch, the EC50 of human PDGFRβ-Fch is 0.05-0.2 μg/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, 25 % Glycerol, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: PDGF-BB Homodimer

Andere Bezeichnung: PDGF-BB

Synonyme: PDGF-2 Protein, PDGF2 Protein, SIS Protein, SSV Protein, c-sis Protein, PDGF-B Protein, Sis Protein, platelet derived growth factor subunit B Protein, platelet derived growth factor, B polypeptide Protein, PDGFB Protein, Pdgfb Protein

Hintergrund:

Background: Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.

Synonym: Platelet-Derived Growth Factor Subunit B, PDGF Subunit B, PDGF-2, Platelet-Derived Growth Factor B Chain, Platelet-Derived Growth Factor Beta Polypeptide, Proto-Oncogene c-Sis, Becaplermin, PDGFB, PDGF2, SIS

Molekulargewicht: 14.3 kDa

NCBI Accession: NP_148937