TXN Protein

Produktnummer ABIN7198219

Produktdetails

Target: TXN (Thioredoxin (TXN))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Verwendungszweck: Recombinant Human Thioredoxin/TXN Protein (Active)

Sequenz: Met 1-Val 105

Produktmerkmale: A DNA sequence encoding the C-terminal segment of human TXN (P10599) (Met 1-Val 105) was expressed and purified.

Reinheit: > 97 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Measured by its ability to catalyze the reduction of insulin. The specific activity is 5-9 pmoles/min/μg.2. Measured by its ability to catalyze the reduction of insulin. The reaction leads toprecipitation, which can be measured by absorbance at 650 nm. The specific activity is 5-10 A650/min/mg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.5

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: TXN (Thioredoxin (TXN))

Andere Bezeichnung: Thioredoxin/TXN (TXN Produkte)

Hintergrund:

Background: Thioredoxin; also known as ATL-derived factor; Surface-associated sulphydryl protein; SASP and TXN; is a nucleus; cytoplasm and secreted protein which belongs to the thioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins; and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO); and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Synonym: Thioredoxin; Trx; ATL-Derived Factor; ADF; Surface-Associated Sulphydryl Protein; SASP; TXN; TRDX; TRX; TRX1

Molekulargewicht: 11.7 kDa

UniProt: P10599

Pathways: Carbohydrate Homeostasis, Cell RedoxHomeostasis