HLA-B*35:01 protein (SBP Tag)

Produktnummer ABIN7273195

Produktdetails

Target: HLA-B*35:01

Protein-Typ: Recombinant

Spezies: Human

Quelle: Mammalian Cells

Aufreinigungstag / Konjugat: SBP Tag

Applikation: Blocking Peptide (BP), Control Peptide (CP), ELISA, Neutralization (Neut)

Verwendungszweck: The product is HLA-B*35:01 complexed with β2-microglobulin (b2m)

Sequenz: heavy chain: GSHSMRYFYT AMSRPGRGEP RFIAVGYVDD TQFVRFDSDA ASPRTEPRAP WIEQEGPEYW DRNTQIFKTN TQTYRESLRN LRGYYNQSEA GSHIIQRMYG CDLGPDGRLL RGHDQSAYDG KDYIALNEDL SSWTAADTAA QITQRKWEAA RVAEQLRAYL EGLCVEWLRR YLENGKETLQ RADPPKTHVT HHPVSDHEAT LRCWALGFYP AEITLTWQRD GEDQTQDTEL VETRPAGDRT FQKWAAVVVP SGEEQRYTCH VQHEGLPKPL TLRWEPSSQS TIPI
light chain: IQRTPKIQVY SRHPAENGKS NFLNCYVSGF HPSDIEVDLL KNGERIEKVE HSDLSFSKDW SFYLLYYTEF TPTEKDEYAC RVNHVTLSQP KIVKWDRDM
Sequences without tags. Tag location is at the discretion of the manufacturer.

Spezifität: HLA-B*35:01 complexed with b2m, P01889 / P61769
HLA-B35 is frequently found in the Caucasoid and the Amerindian major ethnic group, but also in Orientals and Blacks. HLA-B*35:01 is recognized by anti-Bw6 antibodies.

Produktmerkmale: HLAs are produced in mammalian cell cultures as recombinant, truncated, soluble antigens, in complex with β2-microglobulin (HLA class I). As part of our quality control, all HLA are analyzed for purity by gel electrophoresis, concentration is determined by ELISA (0.5 mg/ml) and their functionality is verified with a specific antiserum.

Aufreinigung: Chromatography

Reinheit: > 80 %

Endotoxin-Niveau: Endotoxins have not been removed

Anwendungsinformationen

Applikationshinweise: ELISA: 1 - 100 ng/mL

Kommentare:

sandwich ELISA, direct ELISA, bead ELISA, reactive with antibodies W6/32 and BBM.1 and specific antiserum, B cell ELI Spot (predicted), in vitro memory B cell stimulation (predicted), HLA competition assay (predicted), control in various assays (predicted)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: TRIS based buffer, pH 8.0, Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 6 months

Antigendetails

Target: HLA-B*35:01

Hintergrund: Human Leukozyte Antigen, human Antigen-presenting major histocompatibility complex class I (MHCI) molecule. Expressed on all cells, displays peptides to CD8-positive T cells. Involved in cellular immune response.

Molekulargewicht: approx. 50 kDa (38 kDa heavy chain + 12 kDa b2m)

UniProt: P01889