Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
AMIGO2
(Adhesion Molecule with Ig-Like Domain 2 (AMIGO2))
AMIGO2 Protein, AI415330 Protein, AMIGO-2 Protein, AW208913 Protein, Ali1 Protein, ALI1 Protein, DEGA Protein, adhesion molecule with Ig like domain 2 Protein, adhesion molecule with Ig-like domain 2 Protein, AMIGO2 Protein, Amigo2 Protein
Hintergrund
Bromodomain and extraterminal domain inhibitors (BETi) represent promising therapeutic agents for metastatic melanoma, yet their mechanism of action remains unclear. Here we interrogated the transcriptional effects of BETi and identified AMIGO2, a transmembrane molecule, as a BET target gene essential for melanoma cell survival. AMIGO2 is upregulated in melanoma cells and tissues compared to human melanocytes and nevi, and AMIGO2 silencing in melanoma cells induces G1/S arrest followed by apoptosis.
Molekulargewicht
41.97 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.